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Permit’s take a look at dehydration procedure, making use of xylene-free of charge protocol showcasing isopropyl Alcoholic beverages or IPA. In your xylene-no cost protocols you'll probably have two steps of 80/twenty ethanol/isopropanol mix accompanied by a few techniques of complete isopropanol. Given that Xylene is a relatively harmful solvent

The incubation time in liquid paraffin really should be prolonged more than enough to obtain adequate infiltration, although not any more than essential so as to protect against extended publicity of the tissue to heat. For a similar explanation, it is recommended the temperature on the liquid paraffin should be not more than 2–four C higher than